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Staff

Dr hab. Ewa Dubas

Associate Professors
Department of Cell Biology

Mechanizmy regulacji procesu gametogenezy, embriogenezy niezygotycznej (androgenezy) oraz rozwoju zarodka (cykl komórkowy, cytoszkielet, podłoże hormonalne i (epi)genetyczne, polarność zarodka, programowana śmierć) roślin użytkowych • podwojone haploidy • patogeneza

(+48) 12 4253301 (wew./ext. 39)

DIPLOMAS, DEGREES

2015- present Associate Professor in Agriculture, Agriculture University, Kraków, Poland
2007- PhD in Biology, Jagiellonian University, Kraków, Poland
2001- M.Sc in Biology – Department of Plant Cytology and Embryology, Jagiellonian University, Krakow, Poland

EMPLOYMENT IN ACADEMIC INSTITUTIONS

2022-2024

Deputy Director for Scientific Research in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow

2020-present

Associate Professor, Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow

2020-2020

Adiunct [Assistant Professor], Department of Plant Cytology and Embryology, Jagiellonian University, Krakow

2015-2019

Associate Professor, Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow

2007-2015

Adiunct [Assistant Professor], Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow

2001-2007

PhD student, Department of Cell Biology in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow

2001-2007

Interdisciplinary Ph.D. Study in the Faculty of Biology and Earth Sciences, Jagiellonian University

MEMBERSHIP AND FUNCTIONS

2022-now Deputy Director for Scientific Research in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow
2020-now Member of the Scientific Council in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow
2019-2020 Member of the Scientific Council of the Faculty of Biology of the Jagiellonian University in Krakow 
2015-2019 Member of the Scientific Council in The Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences in Krakow
2014-2017 Polish deputy in Management Committee of COST FA1306 ‘The quest for tolerant varieties: 'Phenotyping at plant and cellular level’
2014 External expert evaluator of the international research projects Fund for Scientific Research – FNRS; Slovak Research and Development Agency; Czech National Agency
2011-2013 Polish deputy in Management Committee of COST FA (Food and Agriculture) 0903 ‘Harnessing Plant Reproduction for Crop Improvement’

RESEARCH AREAS

  • Cell biology (cytoskeleton, cell cycle, auxin and abscisic acid signaling, plasma membrane fluidity, cell death) in responses to abiotic and biotic stress.

  • Effect of stress on plant’s responses in gene and protein levels, transgenesis, plant-growth regulators, in vitro tissue culture of cereals and Brassicaceae.

  • Pathogenesis - cytological and physiological analyses of the infection processes.

PRACTICAL SKILLS

  • In vitro culture: oil-seed rape microspore culture, cereal anther and microspore cultures, immature embryo culture.

  • Tracing of molecules in plant material: analysis of alfa-tubulin, actin, auxin, and auxin efflux carrier proteins localization by immunocytochemistry, fluorescent and confocal laser scanning microscopy (CLSM).

  • Modeling of cellular processes: Graphical Visualization of Cellular Processes in Image Pro-Premier

  • Tracing of the fungal infection process in plant material by cytology and microscopy.

  • Protein analyses: immunolocalisation of proteins in intact cells ('whole mount') and in sectioned material, protein isolation electrophoresis and western blotting.

  • Gene expression: RNA, DNA and pDNA isolation, PCR, qPCR, primer design.

  • Restriction analysis.

  • Bioinformatics: Sequence analysis, gene, and protein sequence alignment: BLAST.

  • Chromosomes analyses: karyotype analyses, C-banding of chromosomes.

  • Quantitative and qualitative analysis of ROS and antioxidative enzyme activity:  fluorescence and fluorometric measurements.

  • Microspores/Androgenic embryos transformation with Agrobacterium tumefaciens.

RESEARCH EXPERIENCE

Post-doc projects. Post-doc projects. My postdoctoral projects focused on: (1) endogenous auxins as inducers of double haploid (DH) production in oilseed rape, (2) the role of polar auxin transport and auxin signalling in embryo development. Plant embryo development involves differential distribution (gradients) of the plant signalling molecule auxin. These gradients are established and maintained by a directional, intercellular auxin transport called polar auxin transport. Polar auxin transport provides positional and directional information for many aspects of embryo development. The B. napus microspore suspension is a suitable model system for such studies. Using a combination of genetic, molecular, physiological, and cell biological approaches, I aim to address the key question of how plant hormones play their respective roles in embryo development, with a particular focus on hormone interactions.

 

PhD project. My PhD project focused on early embryo development in in vitro microspore suspension cultures of Brassica napus. High yields of microspore-derived embryos (MDE) in suspension were reproducibly obtained using the method previously developed by the research team of Dr Jan Custers PRI Wageningen, The Netherlands. I have adopted this method and further optimised it in my laboratory. MDE develop and differentiate, in a manner similar to zygotic embryos in almost every aspect studied, so I used them to study the early stages of plant embryogenesis. The main aim of these studies was to study the development of androgenic embryos from the earliest stages (a single cell), including suspensor formation. The study of these structures during zygotic development is very difficult due to the ovarian tissues surrounding the embryos. I focused on the establishment of embryo polarity and the role of the cytoskeleton during MDE differentiation, also tracking the positions of mitochondria and amyloplasts, vacuolization patterns, cell wall formation, and differentiation of embryonic organs. My studies required the optimisation of many methods and the development of protocols for immunodetection and tracking of different structures in whole-mount (not sectioned) material.

            

 

PUBLICATIONS

                   

 

Selected older papers (up to 2017) listed by year with links to the publishers' websites:

2015-2018

  1. Golebiowska-Pikania G., Kopeć P., Surówka E., Janowiak F., Krzewska M., Dubas E., Nowicka A., Kasprzyk J., Ostrowska A., Malaga S., Hura T., Żur I. 2017. Changes in protein abundance and activity induced by drought during generative development of winter barley (Hordeum vulgare L.). Journal of Proteomics 169:73-86, doi: 10.1016/j.jprot.2017.07.016 Strona wydawcy 
  2. Golebiowska-Pikania G., Kopeć P., Surówka E., Krzewska M., Dubas E., Nowicka A., Rapacz M., Wójcik-Jagła M., Malaga S., Żur I. 2017. Changes in protein abundance and activity involved in freezing tolerance acquisition in winter barley (Hordeum vulgare L.). Journal of Proteomics 169:58-72, doi: 10.1016/j.jprot.2017.08.019 Strona wydawcy
  3. Krzewska M., Gołębiowska-Pikania G., Dubas E., Gawin M., Żur I. 2017. Identification of proteins related to microspore embryogenesis responsiveness in winter triticale (×Triticosecale Wittm.). Euphytica 213:192. doi: 10.1007/s10681-017-1978-1 Strona wydawcy
  4. Śniegowska-Świerk K., Dubas E. & Rapacz M. 2016. Actin microfilaments are involved in the regulation of HVA1 transcript accumulation in drought-treated barley leaves. Journal of Plant Physiology, 193, 22-25. Strona wydawcy
  5. Wędzony M., Żur I., Krzewska M., Dubas E., Szechyńska-Hebda M., Wąsek I. 2015. Doubled haploids in triticale. Eudes F. (ed.), Triticale, Springer, pp.111-128. Strona wydawcy
  6. Krzewska M., Czyczyło-Mysza I., Dubas E., Gołębiowska-Pikania G., Żur I. 2015. Identification of QTLs associated with albino plant formation and some new facts concerning green versus albino ratio determinants in triticale (×Triticosecale Wittm.) anther culture. Euphytica, 1-16. doi: 10.1007/s10681-015-1509-x Strona wydawcy
  7. Żur I., Dubas E., Krzewska M., Waligórski P., Dziurka M., Janowiak F. 2015. Hormonal requirements for effective induction of microspore embryogenesis in triticale (×Triticosecale Wittm.) anther cultures. Plant Cell Rep. 34(1):47-62. Strona wydawcy
  8. Żur I., Dubas E., Krzewska M., Janowiak F. 2015. Current insights into hormonal regulation of microspore embryogenesis. Frontiers in Plant Science 6 doi:10.3389/fpls.2015.00424 Strona wydawcy
  9. Żur I., Dubas E., Krzewska M., Waligórski P.,  Dziurka M.,  Janowiak F. 2015. Hormonal requirements for effective induction of microspore embryogenesis in triticale (× Triticosecale Wittm.) anther cultures. Plant Cell Reports, 34:47-62, doi:10.1007/s00299-014-1686-4 Strona wydawcy

  1. Dubas E., Moravčíková J., Libantová J., Matušíková I., Benkova E., Żur I., Krzewska M. 2014. The influence of heat stress on auxin distribution in transgenic B. napus microspores and microspore derived embryos. Protoplasma 251:1077-1087. IF2013 3.171  Read more

  2. Dubas E., Custers J., Kieft H., Wędzony M., van Lammeren A.A.M. 2014. Characterization of polarity development through 2- and 3-D imaging during the initial phase of microspore embryogenesis in Brassica napus L. Protoplasma 251: 103-113. IF2013 3.171  Read more

  3. Wędzony M., Szechyńska-Hebda M., Żur I., Dubas E., Krzewska M. 2014. Tissue culture and regeneration: a prerequisite for alien gene transfer. In: Alien Gene Transfer in Crop Plants, Vol. 1. Innovations, Methods and Risk Assessment. Pratap A., Kumar, J. (Eds.), Springer, ISBN 978-1-4614-8584-1 Read more

  4. Żur I., Dubas E., Krzewska M., Janowiak F., Hura K., Pociecha E., Bączek-Kwinta R., Płażek A. 2014. Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis. Plant Cell, Tissue and Organ Culture (PCTOC). DOI: 10.1007/s11240-014-0515. IF2013: 2.61  Read more

  5. Żur I.,.Dubas E*., Krzewska M., Sánchez-Díaz R.A., Castillo A.M., Vallés M.P. 2014. Changes in gene expression patterns associated with microspore embryogenesis in hexaploid triticale (× Triticosecale Wittm.)", submitted to Plant Cell, Tissue and Organ Culture (PCTOC) 116(2):261-267. IF2013 2.612  Read more

  6. Żur I.,.Dubas E., Krzewska M., Waligórski P., Dziurka M., Janowiak F. 2014. Hormonal requirements for effective induction of microspore embryogenesis in triticale (×Triticosecale Wittm.) anther cultures. Plant Cell Rep DOI 10.1007/s00299-014-1686-4. MNiSW 35 pkt., IF2013 2,936  Strona wydawcy

  7. Dubas E., Janowiak F., Krzewska M., Hura T., Żur I. 2013. Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape (Brassica napus L.) genotypes differing in responsiveness to androgenesis induction. Plant Cell Rep 32:1465–1475. IF2013 2.936  Read more

  8. Pociecha E., Janowiak F., Dubas E., Żur I., Tokarz K., Kolasińska I., Płażek A. 2013. Progress of snow mould infection in crowns of winter rye (Secale cereale L.) is related to photosynthetic activity during cold acclimation. Plant Physiology and Biochemistry 70:360-367. IF2013: 2.352 Read more

  9. Żur I., Gołębiowska G., Dubas E., Golemiec E., Matušíková I., Libantová J., Moravčíková J. 2013. β-1,3-glucanase and chitinase activities in winter triticales during cold hardening and subsequent infection by Microdochium nivale. Biologia 68/2: 241-248. IF2013: 0.696 Read more

  10. Żur I., Dubas E., Słomka A., Dubert F., Kuta E., Płażek A. 2013. Failure of androgenesis in Miscanthus×giganteus in vitro culture of cytologically unbalanced microspores. Plant Reproduction 26(3):297-307. IF2012: 2.07   Read more

  1. Dubas E., Wędzony M., Custers J., Kieft H., van Lammeren A.A.M. 2012. Gametophytic development of Brassica napus pollen in vitro enables examination of cytoskeleton and nuclear movements. Protoplasma 249(2): 369-377. IF2013: 3.171  Read more

  2. Krzewska M., Czyczyło-Mysza I., Dubas E., Gołębiowska-Pikania G., Golemiec E., Stojałowski S., Chrupek M., Żur I. 2012. Quantitative trait loci associated with androgenic responsiveness in triticale (×Triticosecale Wittm.) anther culture. Plant Cell Reports 31:2099–2108. Plant Cell Reports 31:2099–2108. IF2013: 2.936  Read more

  3. Słomka A., Kuta E., Płażek A., Dubert F., Żur I., Dubas E., Kopeć P., Żurek G. 2012. Sterility of Miscanthus×giganteus results from hybrid incompatibility. Acta Biologica Cracoviensia Series Botanica 54/1: 1–8. IF2013: 0.662 Read more

  4. Żur I., Krzewska M., Dubas E., Gołębiowska-Pikania G., Janowiak F., Stojałowski S. 2012. Molecular mapping of loci associated with abscisic acid accumulation in triticale (×Triticosecale Wittm.) anthers in response to low temperature stress inducing androgenic development. Plant Growth Regulation 68(3): 483-492. IF2013: 1.625  Read more

  5. Dubas E., Custers J.B.M., Kieft H., Wędzony M., van Lammeren  A.A.M. 2011. Microtubule configurations and nuclear DNA synthesis in microspore derived embryos of Brassica napus cv Topas that mimic zygotic embryo development. Plant Cell Reports 30: 2105–2116. IF2013 : 2.936   Read more

  6. Dubas E., Golebiowska G., Zur I., Wedzony M. 2011. Microdochium nivale (Fr., Samuels & Hallett): cytological analysis of the infection process in triticale (x Triticosecale Wittm.). Acta Physiologiae Plantarum 33(2): 529-537. IF2013: 1.524  Read more

  7. Żur I.A., Dubas E., Pociecha E., Dubert F., Kolasińska I., Płażek A. 2011. Cytological analysis of infection process and the first defence responses induced in winter rye (Secale cereale L.) seedlings inoculated with Microdochium nivale. Physiological and Molecular Plant Pathology 76: 189-196. IF2013: 1.987 Read more

  8. Szechyńska-Hebda M., Wędzony M., Tyrka M., Gołębiowska G., Chrupek M., Czyczyło-Mysza I., Dubas E., Żur I., Golemiec E. 2011. Identifying QTL for cold-induced resistance to Microdochium nivale in winter triticale. Plant Genetic Resources: Characterization and utilization 9(2):296-299. IF2013: 1.057 Read more

  1. Dubas E., Wędzony M., Petrovska B., Salaj J., Żur I. 2010. Cell structural reorganization during induction of androgenesis in isolated microspore cultures of triticale (x Triticosecale Wittm.). Acta Biologica Cracoviensia Series Botanica 52/1: 73–86. IF2013: 0.662 Read more

  2. Dubas E., Marzec K., Płażek A. 2010. Cytological studies on Microdochium nivale infection in Lolium perenne (L.) plants. Zeszyty Problemowe Postępów Nauk Rolniczych PAN Zeszyt 545:389-401.

  3. Żur I., Dubas E., Golemiec E., Szechyńska-Hebda M., Gołębiowska G., Wędzony M. 2009. Stress-related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (xTriticosecale Wittm.). Plant Cell Reports 28:1279–1287. IF2013: 2.36  Read more

  4. Wędzony M., Forster B.P., Żur I., Golemiec E., Szechyńska-Hebda M., Dubas E., Gołębiowska G. 2009.  Progress in Doubled Haploid Technology in Higher Plants. In: Touraev A., Jain M., Forster B. (eds.): “Advances in Haploid Production in Higher Plants” © Springer Science + Business Media B.V., pp.1-35. IC 30 Read more

  1. Supena EDJ., Winarto B., Riksen T., Dubas E., van Lammeren A., Offringa R., Boutilier K., Custers J. 2008. Regeneration of zygotic-like microspore-derived embryos suggests an important role for the suspensor in early embryo patterning. Journal of Experimental Botany 59(4): 803-814. doi:10.1093/jxb/erm358.IF2013: 5.794  Read more

  2. Żur I., Dubas E., Golemiec E., Szechyńska-Hebda M., Janowiak F., Wędzony M. 2008. Stress-induced changes important for effective androgenic induction in isolated microspore culture of triticale (xTriticosecale Wittm.). Plant Cell, Tissue and Organ Culture 94:319-328.  Read more

  3. Szechyńska-Hebda M., Wędzony M., Dubas E., Kieft H., van Lammeren A. 2006. Visualisation of microtubules and actin filaments in fixed BY-2 suspension cells using an optimized whole mount immunolabeling protocol. Plant Cell Reports. 25 (8): 758 – 766. IF2013: 2.936  Read more

  4. Żur I., Godzisz I., Dubas E., Golemiec E., Wędzony M. 2006. The influence of cold pretreatment on androgenesis efficiency in isolated microspore culture of triticale (X Triticosecale Wittm.). Zeszyty Problemowe Postępów Nauk Rolniczych 509: 49-59.

 

COORDINATION/PARTICIPATION IN RESEARCH PROJECTS 

NATIONAL  (RUNNING PROJECTS)

2021 - 2026 ‘Identification of factors determining the efficiency of microspore embryogenesis induction in wheat (Triticum aestivum L.)’ research project financed by Ministry of Agriculture and Rural Development, Task No. No 4; Host Institution: Polish Academy of Sciences The Franciszek Górski Institute of Plant Physiology in Kraków; a role: researcher

NATIONAL  (FINISHED PROJECTS)

2016 - 2021 'Oxidative stress and its role in the regulation of embryogenesis in isolated microspore cultures of triticale (xTriticosecale Wittm.) and barley (Hordeum vulgare L.)' research project financed by National Science Centre; 2015/18/M/NZ3/00348; a role: researcher
2017 - 2020 ‘Protocol for effective doubled haploids production by androgenesis for Polish breeding materials of wheat (Triticum aestivum L.)’ research project financed by National Centre of Research and Development; Tango2/340285/NCBR/2017; a role: researcher
2015 - 2018 ‘Identification of factors determining the efficiency of doubled haploids production in rye (Secale cereale L.) through androgenesis and distant crosses’ research project financed by Ministry of Agriculture and Rural Development, Task No. 84; Host Institution: Polish Academy of Sciences The Franciszek Górski Institute of Plant Physiology in Kraków; a role: project coordinator
2014 - 2018 ‘Identification of factors determine drought and frost resistance in winter barley (Hordeum vulgare L.)’ research project. Biological progress, Task No. 26 (Appendix No. 9 to the Regulation of the Minister of Agriculture and Rural Development on the rate subsidy for various entities performing tasks for agriculture in May 18th, 2010 (Journal of Laws No. 91, Item 595, as amended); a role: researcher
2013 - 2014 ‘Identification of drought induced signalling pathway in barley leaves’. Research national project No. G-1843 ; a role: researcher
2011 - 2014  ‘The role of endogenous auxins: Indole-3-acetic acid (IAA) and Indole-3-butyric acid (IBA) in auxin homeostasis and androgenic effectivness enhancement in oilseed rape (Brassica napus)’. Individual research national project No. 2011/01/D/NZ9/02547; a role: project coordinator

 BILATERAL (FINISHED PROJECTS)

2016 - 2018 ‘Stress-inducible expression of arabinogalactans and defence-related proteins in relation to androgenesis initiation’. Polish - Slovak research project (Host Institutions: IPP PAS and Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences); a role: project coordinator.
2016 - 2018 ‘Chitinases and AGPs as proteins diversifying genotypes with high ability to microspore embryogenesis from non-embryogenic in crops’. CHIAGP, No. SK-PL-2015-0044. Polish – Slovak research project (Host Institutions: IPP PAS and Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences); a role: project coordinator.
Springer A., Krzewska M., Dubas E., Kopeć P., Plačková L., Doležal K., Weigt D., Żur I.

Induction of microspore embryogenesis in bread wheat by mannitol pre-treatment is associated with the disruption of endogenous hormone balance and substantial accumulation of auxins

BMC Plant Biology, 25: 370 2025

More
Nowicka A., Kovacik M., Maksylewicz A., Kopeć P., Dubas E., Krzewska M., Springer A., Hoffie R.E., Daghma D.S., Milec Z., Pecinka A., Kumlehn J., Żur I.

The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley

The Crop Journal, 12: 1064-1080 2024

More
Żur I., Adamus A., Cegielska-Taras T., Cichorz S., Dubas E., Gajecka M., Juzoń-Sikora K., Kiełkowska A., Malicka M., Oleszczuk S., Skrzypek E., Szała L., Szarejko I., Zimny J.

Doubled haploids: Contributions of poland’s academies in recognizing the mechanism of gametophyte cell reprogramming and their utilization in breeding of agricultural and vegetable species

Acta Societatis Botanicorum Poloniae, 91: 9128 2022

More
Dubas E., Castillo A.M., Żur I., Krzewska M., Vallés M.P.

Microtubule organization changes severely after mannitol and n-butanol treatments inducing microspore embryogenesis in bread wheat

BMC Plant Biology, 21: 586 2021

More
Dubas E., Żur I., Moravčiková J., Fodor J., Krzewska M., Surówka E., Nowicka A., Gerši Z.

Proteins, small peptides and other signaling molecules identified as inconspicuous but possibly important players in microspores reprogramming toward embryogenesis

Frontiers in Sustainable Food Systems, 5: 745865 2021

More
Żur I., Gajecka M., Dubas E., Krzewska M., Szarejko I.

Albino plant formation in androgenic cultures: An old problem and new facts

Segui-Simarro J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2288. Humana, New York, NY 2021

More
Zieliński K., Dubas E., Gerši Z., Krzewska M., Springer A., Nowicka A., Matušíková I., Żur I., Sakuda S., Moravčíková J.

β-1,3-glucanases and chitinases participate in the stress-related defence mechanisms that are possibly connected with modulation of arabinogalactan proteins (AGP) required for the androgenesis initiation in rye (Secale cereale L.)

Plant Science, 302: 110700 2021

More
Sychta K., Dubas E., Yamada K., Słomka A., Krzewska M., Kuta E.

Papain-like cysteine proteases are involved in programmed cell death in plants under heavy metal stress

Environmental and Experimental Botany, 174: 104041 2020

More
Żur I., Dubas E., Krzewska M., Zieliński K., Fodor J, Janowiak F.

Glutathione provides antioxidative defence and promotes microsporederived embryo development in isolated microspore cultures of triticale (×Triticosecale Wittm.)

Plant Cell Reports, 38: 195–209 2019

More
Gołębiowska-Pikania G., Kopeć P., Surówka E., Krzewska M., Dubas E., Nowicka A., Rapacz M., Wójcik-Jagła M., Malaga S., Żur I.

Changes in protein abundance and activity involved in freezing tolerance acquisition in winter barley (Hordeum vulgare L.)

Journal of Proteomics, 169: 58-72 2017

More
Krzewska M., Gołębiowska-Pikania G., Dubas E., Gawin M., Żur I.

Identification of proteins related to microspore embryogenesis responsiveness in anther cultures of winter triticale (×Triticosecale Wittm.)

Euphytica, 213 :192 2017

More
Śniegowska-Świerk K., Dubas E., Rapacz M.

Actin microfilaments are involved in the regulation of HVA1 transcript accumulation in drought-treated barley leaves

Journal of Plant Physiology, 193: 22-25 2016

More
Głąb T., Szewczyk W., Dubas E., Kowalik K., Jezierski T.

Anatomical and morphological factors affecting wear tolerance of turfgrass

Scientia Horticulturae, 185: 1-13 2015

More
Śniegowska-Świerk K., Dubas E., Rapacz M.

Drought-induced changes in the actin cytoskeleton of barley (Hordeum vulgare L.) leaves

Acta Physiologiae Plantarum, 37: 73 2015

More
Krzewska M., Czyczyło-Mysza I., Dubas E., Gołębiowska-Pikania G., Żur I.

Identification of QTLs associated with albino plant formation and some new facts concerning green versus albino ratio determinants in triticale (×Triticosecale Wittm.) anther culture

Euphytica, 206: 263-278 2015

More
Szechyńska-Hebda M., Wąsek I., Gołębiowska-Pikania G., Dubas E., Żur I., Wędzony M.

Photosynthesis-dependent physiological and genetic crosstalk between cold acclimation and cold-induced resistance to fungal pathogen in triticale (×Triticosecale Wittm.)

Journal of Plant Physiology, 177: 30-43 2015

More
Żur I., Dubas E., Krzewska M., Janowiak F., Hura K., Pociecha E., Bączek-Kwinta R., Płażek A.

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Plant Cell Tissue and Organ Culture, 119: 79-94 2014

More
Dubas E., Custers J., Kieft H., Wędzony M., van Lammeren A.A.M.

Characterization of polarity development through 2- and 3-D imaging during the initial phase of microspore embryogenesis in Brassica napus L.

Protoplasma, 251: 103-113 2014

More
Dubas E., Moravčíková J., Libantová J., Matušíková I., Benkova E., Żur I., Krzewska M.

The influence of heat stress on auxin distribution in transgenic B. napus microspores and microspore derived embryos

Protoplasma, 251:1077-1087 2014

More
Wędzony M., Szechyńska-Hebda M., Żur I., Dubas E., Krzewska M.

Tissue culture and regeneration: a prerequisite for alien gene transfer

In: Alien Gene Transfer in Crop Plants, Vol. 1. Innovations, Methods and Risk Assessment. Pratap A., Kumar, J. (eds), Springer, Dordrecht, Heidelberg, London, New York: 43-75 2014

More
Żur I., Dubas E., Krzewska M., Sánchez-Díaz R.A., Castillo A.M., Vallés M.P.

Changes in gene expression patterns associated with microspore embryogenesis in hexaploid triticale (× Triticosecale Wittm.)

Plant Cell, Tissue and Organ Culture, 116: 261-267 2014

More
Żur I., Gołębiowska-Pikania G., Dubas E., Golemiec E., Matušíková I., Libantová J., Moravčíková J.

β-1,3-glucanase and chitinase activities in winter triticales during cold hardening and subsequent infection by Microdochium nivale

Biologia, 68: 241-248 2013

More
Żur I., Dubas E., Słomka A., Dubert F., Kuta E., Płażek A.

Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores

Sexual Plant Reproduction, 26: 297-307 2013

More
Pociecha E., Janowiak F., Dubas E., Żur I., Tokarz K., Kolasińska I., Płażek A.

Progress of snow mould infection in crowns of winter rye (Secale cereale L.) is related to photosynthetic activity during cold acclimation

Plant Physiology and Biochemistry, 70: 360-367 2013

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Dubas E., Janowiak F., Krzewska M., Hura T., Żur I.

Endogenous ABA concentration and cytoplasmic membrane fluidity in microspores of oilseed rape (Brassica napus L.) genotypes differing in responsiveness to androgenesis induction

Plant Cell Reports, 32: 1465-1475 2013

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Żur I., Krzewska M., Dubas E., Gołębiowska-Pikania G., Janowiak F., Stojałowski S.

Molecular mapping of loci associated with abscisic acid accumulation in triticale (x Triticosecale Wittm.) anthers in response to low temperature stress inducing androgenic development

Plant Growth Regulation, 68: 483-492 2012

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Słomka A., Kuta E., Płażek A., Dubert F., Żur I., Dubas E., Kopeć P., Żurek G.

Sterility of Miscanthus x Giganteus results from hybrid incompatibility

Acta Biologica Cracoviensia. Series Botanica 54: 1-8 2012

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Krzewska M., Czyczyło-Mysza I., Dubas E., Gołębiowska-Pikania G., Golemiec E., Stojałowski S., Chrupek M., Żur I.

Quantitative trait loci associated with androgenic responsiveness in triticale (x Triticosecale Wittm.) anther culture

Plant Cell Reports, 31: 2099-2108 2012

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Dubas E., Wędzony M., Custers J., Kieft H., van Lammeren A.A.M.

Gametophytic development of Brassica napus pollen in vitro enables examination of cytoskeleton and nuclear movements

Protoplasma, 249: 369-377 2012

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Żur I., Dubas E., Pociecha E., Dubert F., Kolasińska I., Płażek A.

Cytological analysis of infection process and the first defence responses induced in winter rye (Secale cereale L.) seedlings inoculated with Microdochium nivale

Physiological and Molecular Plant Pathology, 76: 189-196 2011

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Dubas E., Gołębiowska G., Żur I., Wędzony M.

Microdochium nivale (Fr., Samuels & Hallett): cytological analysis of the infection process in triticale (x Triticosecale Wittm.)

Acta Physiologiae Plantarum, 33: 529-537 2011

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Dubas E., Custers JBM., Kieft H., Wędzony M., van Lammeren A.A.M.

Microtubule configurations and nuclear DNA synthesis in microspore derived embryos of Brassica napus cv Topas that mimic zygotic embryo development

Plant Cell Reports, 30: 2105-2116 2011

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Dubas E., Marzec K., Płażek A.

Cytological studies on Microdochium nivale infection in Lolium perenne (L.) plants

Zeszyty Problemowe Postępów Nauk Rolniczych, 545: 389-401 2010

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Dubas E., Wędzony M., Petrovska B., Salaj J., Żur I.

Cell structural reorganization during induction of androgenesis in isolated microspore cultures of triticale (x Triticosecale Wittm.)

Acta Biologica Cracoviensia. Series Botanica, 52: 73-86 2010

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Dubas E., Custers J., Lammeren A., Kieft H., Żur I., Wędzony M.

Brassica napus L. microspore culture: auxin efflux carrier proteins

Annual Report. Polish Academy of Sciences: 69-70 2009

Wędzony M., Forster B.P., Żur I., Golemiec E., Szechyńska-Hebda M., Dubas E., Gołębiowska G.

Progress in doubled haploid technology in higher plants

In: Advances in haploid production in higher plants. Touraev A., Forster B.P., Jain S.M. (eds), Springer Science + Business Media B.V.: 1-34 2009

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Żur I., Dubas E., Golemiec E., Szechyńska-Hebda M., Gołębiowska G.

Stress-related variation in antioxidative enzymes activity and cell metabolism efficiency associated with embryogenesis induction in isolated microspore culture of triticale (x Triticosecale Wittm.)

Plant Cell Reports, 28: 1279-1287 2009

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Żur I., Dubas E., Golemiec E., Szechyńska-Hebda M., Janowiak F., Wędzony M.

Stress-induced changes important for effective androgenic induction in isolated microspore culture of triticale (× Triticosecale Wittm.)

Plant Cell, Tissue and Organ Culture, 94: 319-328 2008

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Supena E.D., Winarto B., Riksen T., Dubas E., van Lammeren A., Offringa R., Boutilier K., Custers J.

Regeneration of zygotic-like microspore-derived embryos suggests an important role for the suspensor in early embryo patterning

Journal of Experimental Botany, 59: 803-814 2008

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Żur I., Godzisz I., Dubas E., Golemiec E., Wędzony M.

The influence of cold pretreatment on androgenesis efficiency in isolated microspore culture of triticale (X Triticosecale WITTM.)

Zeszyty Problemowe Postepow Nauk Rolniczych 2006

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Szechyńska-Hebda M., Wędzony M., Dubas E., Kieft H., van Lammeren A.

Visualisation of microtubules and actin filaments in fixed BY-2 suspension cells using an optimised whole mount immunolabelling protocol

Plant Cell Reports, 25: 758-766 2006

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Płażek A., Dubas E.

Activation of phenylpropanoid pathway and catalase in leaves of barley and meadow fescue infected by Bipolaris sorokiniana (Sacc.) Shoem

In: Recent advances in genetics and breeding of the grasses. Zwierzykowski Z., Kosmala A. (eds), PAGEN, Monografie Instytutu Genetyki Roślin PAN w Poznaniu, 197-202 2005

Lammeren A.A., Wędzony M., Dubas E., Szechyńska-Hebda M.

Fluorescent microscopy to study plant response to stress

In: Analytical methods in plant stress biology. Filek M., Biesaga-Kościelniak J., Marcińska I. et al. (eds), Institute of Plant Physiology, PAS, Kraków: 133-142 2004

Żur I., Dubas E., Golemiec E., Wędzony M., Sabatowska M.

Stress as a trigger for androgenesis induction in isolated microspore culture of wheat (Triticum aestivum L.)

Zeszyty Problemowe Postępów Nauk Rolniczych, 496: 591-599 2004

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Projects

Regulation of Glandular Trichome Metabolism by New Genomic Techniques (NGT)

Nr projektu: DWD/8/0369/2024

Finansowanie: MNiSW

Rok rozpoczęcia: 2024

Rok zakończenia: 2028

Kierownicy projektu: E. Dubas

Wykonawcy: J. Kurczab

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Oxidative stress and its role in the regulation of embryogenesis in isolated microspore cultures of triticale (x Triticale Wittm.) and barley (Hordeum vulgare L.)

Nr projektu: 2015/18/M/NZ3/00348

Finansowanie: National Science Centre

Rok rozpoczęcia: 2016

Rok zakończenia: 2021

Kierownicy projektu: I. Żur

Wykonawcy: E. Dubas, F. Janowiak, M. Krzewska, A. Nowicka, E. Surówka, S. Malaga, K. Zieliński, Jochen Kumlehn, Balazs Barna, Jozsef Fodor, K. Juzoń-Sikora

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Protocol for effective doubled haploids production by androgenesis for polish breeding materials of wheat (Triticum aestivum L.)

Nr projektu: Tango2/340285/NCBR/2017

Finansowanie: National Centre of Research and Development

Rok rozpoczęcia: 2017

Rok zakończenia: 2020

Kierownicy projektu: I. Żur

Wykonawcy: E. Dubas, M. Krzewska, K. Juzoń-Sikora, Maria Wędzony

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The role of endogenous auxins: Indole-3-acetic acid (IAA) and Indole-3-butyric acid (IBA) in auxin homeostasis and androgenic effectivness enhancement in oilseed rape (Brassica napus)

Nr projektu: 2011/01/D/NZ9/02547

Finansowanie: National Science Centre

Rok rozpoczęcia: 2011

Rok zakończenia: 2014

Kierownicy projektu: E. Dubas

Wykonawcy: I. Żur, M. Krzewska, P. Waligórski

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The identification of proteins connected with androgenic responsiveness in triticale (× Triticosecale Wittm.)

Nr projektu: 2011/01/N/NZ9/02541

Finansowanie: National Science Centre

Rok rozpoczęcia: 2012

Rok zakończenia: 2014

Kierownicy projektu: M. Krzewska

Wykonawcy: I. Żur, E. Dubas, G. Gołębiowska-Pikania

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The possibility of increasing the efficiency of androgenesis in triticale (×Triticosecale Wittm) by controlling the hormone balance and intensity of oxidative stress in anthers

Nr projektu: N N310 452638

Finansowanie: National Science Center

Rok rozpoczęcia: 2010

Rok zakończenia: 2013

Kierownicy projektu: I. Żur

Wykonawcy: E. Dubas, F. Janowiak, P. Waligórski

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Localization of QTL for androgenic response on triticale (×Triticosecale Wittm.)

Nr projektu: 548/N-COST/2009/0

Finansowanie: National Science Center, Action COST FA0604 (TRITIGEN)

Rok rozpoczęcia: 2009

Rok zakończenia: 2012

Kierownicy projektu: I. Żur

Wykonawcy: E. Dubas, I. Czyczyło-Mysza, F. Janowiak, M. Krzewska

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Genetic background of triticale resistance to Blumeria graminis

Nr projektu: PBZ-MNiSW-2/3/2006/20

Finansowanie: Ministry of Science and Higher Education

Rok rozpoczęcia: 2007

Rok zakończenia: 2011

Wykonawcy: I. Żur, E. Dubas

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Genetic resistance to fungal-born deceases necessary for sustainable, low input agriculture – genetic and physiologic basis of triticale (xTriticosecale) resistance to snow mould resulted from Microdochium nivale infection

Nr projektu: MNiSW 7/COS/2007/01

Finansowanie: Ministry of Science and Higher Education, COST Action

Rok rozpoczęcia: 2007

Rok zakończenia: 2009

Wykonawcy: I. Żur, E. Dubas

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Improvement and practical application of triticale hybrid breeding technology based on cytoplasmic male-sterility from Triticum timopheevi

Nr projektu: Umowa z dnia 1 maja 2006

Finansowanie: DANKO Plant Breeding Company

Rok rozpoczęcia: 2006

Rok zakończenia: 2007

Wykonawcy: I. Żur, E. Dubas

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