Department of Biotechnology

Leader Prof. dr hab. inż. Ilona Czyczyło-Mysza

Address

ul. Niezapominajek 21,
30-239 Kraków

Contact

telephone: (+48) 12 4251833 (wew./ex. 109)
faks: (+48) 12 4251844
i.czyczylo@ifr-pan.edu.pl

Quantitative traits loci (QTL) • phenotyping • plants' tissue cultures • androgenesis and wide crossing of cereals

Scientific staff

SCIENTIFIC INTRESTS:

Androgenesis and wide crossing of cereals
Endogenous phytohormones regulating the conversion of haploid oat embryos into plants
Physiological and cytogenetic characterization of oat x maize hybrids
Phenotyping and identification of QTLs involved in physiological-biochemical processes, yield and tolerance to abiotic and biotic stresses in crop plants
Genetic, biochemical and physiological determinants of rye response to drought stress with reference to the influence of epicuticular wax structure
Optimization of regeneration methods for chosen endangered species and crop plants, with particular emphasis on legumes and cereals

SCIENTIFIC METHODOLOGY:

In vitro culture techniques: callus induction and regeneration, cell suspension cultures, protoplasts isolation, somatic and gametic embryogenesis, production of cereal doubled haploids
Histological and cytological analyses
Analysis of plant tissues ploidy by flow cytometry (MACS Quant, Miltenyi Biotec)
Hydroponic cultures of chosen crop plants
Plant phenotyping and QTL analysis
Spectrophotometic/chromatographic analysis of phenolics, polyamines, carbohydrates, proteins, plant pigments, antioxidative enzymes activities
Measurement of chlorophyll fluorescence (Handy PEA, Hansatech)
Measurement of chlorophyll content (SPAD, Konica Minolta)
Determination of fatty acids and wax fraction components by gas chromatography

ACHIEVEMENTS:

Obtaining the polish patent no 169824 entitled: Method for culture of fungi containing chitin
Showing that winter wheat callus is not capable of the vernalisation process, however it transfers the ability of generative development into regenerated plants
Detection that the repression of genes responsible for the state of generative induction of winter wheat plants occurs in the course of the process of gamete formation during meiosis
Detection of aromatic cytokinin from BAP group in winter wheat, and correlation of their endogenous content in immature inflorescences and embryos with their ability of generative development
Enriching the map of wheat with 1445 DArT markers, which allowed to dense the map based on SSR, RFLP and AFLP markers for 472 unique loci. Identification of gene candidates for mapped quantitative trait loci (QTLs) of yield components, chlorophyll fluorescence kinetics parameters and photosynthetic pigments
Implementation of oat doubled haploid lines obtained using the wide crossing method in breeding programs
Identification and characterisation of oat x maize hybrids
Poor conversion of oat haploid embryos into plants was due to their immaturity, their irregular morphology and anatomy as well as low content of endogenous auxins suggest disturbances in auxin signaling
Rye's response to drought depends not only on the amount and structure of wax, but also on the interactions between the two, the genetic background of plants, and environmental conditions

AWARDS:

2014 - Distinction of the Minister of Agriculture and Rural Development for outstanding national achievement relevant to the implementation of progress in agricultural practice and realization, implementation and dissemination of the results of scientific work entitled: "Getting the doubled haploid lines of oat (Avena sativa L.) by wide crossing method" awarded to the team of authors: IPP PAS: dr hab. inż. Edyta Skrzypek, prof. dr hab. Izabela Marcińska, dr hab. inż. Ilona Czyczyło-Mysza, dr inż. Kinga Dziurka, dr Agata Nowakowska oraz PB Strzelce Sp. z o.o., Gr. IHAR: dr inż. Zygmunt Nita, inż. Krystyna Werwińska
2021 - Distinction of the Minister of Agriculture and Rural Development granted for outstanding national achievement in the implementation of progress in agriculture and agricultural markets entitled: "Development of a dietary products sets and food products with high pro-health properties" awarded to the IPP PAS team: dr hab. inż. I. Czyczyło-Mysza (team leader), prof. dr hab. inż. E. Pisulewska, prof. dr hab. inż. F. Janowiak, dr hab. P. Waligórski, dr hab. inż. A. Janeczko, mgr inż. K. Laskoś, dr inż. Sz. Polaszczyk, mgr I. Sadura
2022 - Third Degree Young Researcher Award for mgr inż. K. Laskoś awarded by the Polish Botanical Society for the achievement: Laskoś K., CzyczyłoMysza I.M., Dziurka M., Noga A., Góralska M., Bartyzel J., Myśków B. (2021). Correlation between leaf epicuticular wax composition and structure, physiobiochemical traits and drought resistance in glaucous and non-glaucous near-isogenic lines of rye. Plant J, 108: 93-119

Publications

Ptak A., Warchoł M., Morańska E., Laurain-Mattar D., Spina R., Dupire F., Waligórski P., Simlat M. 2026

Amaryllidaceae alkaloids and phenolic acids identification in Leucojum aestivum L. plant cultures exposed to different temperature conditions

Molecules, 31: 258

PDF

Laskoś K., Waligórski P., Pisulewska E., Janowiak F., Sadura-Berg I., Czyczyło-Mysza I. 2025

Herbal maceration modulates fatty acid profile of cold-pressed oils and preserves antioxidant activity during long-term storage

Scientific Reports, 15: 38592

PDF

Dziurka K., Duszyn M., Muszyńska E. 2025

Editorial: Plant Hormone Signaling

International Journal of Molecular Sciences, 26: 10554

PDF

Hura T., Hura K., Ostrowska A., Urban K., Dziurka K., Pawłowska B. 2025

Repeated soil droughts induce differential responses across leaflets of a compound leaf

Plant, Cell and Environment, 48: 8873–8877

PDF

Warzecha T., Warchoł M., Bathelt R., Bocianowski J., Idziak-Helmcke D., Sutkowska A., Skrzypek E. 2025

Evaluation of drought tolerance in oat × maize addition lines through biochemical and yield traits

Agronomy, 15: 2259

PDF

Dziurka K., Dziurka M., Sujkowska-Rybkowska M., Laskoś K., Grela M., Muszyńska E. 2025

Auxin metabolite balance during haploid and zygotic oat embryo development—quantitative and localization studies

International Journal of Molecular Sciences, 26: 5737

PDF

Grela M., Quarrie S., Cyganek K., Bocianowski J., Karbarz M., Tyrka M., Habash D., Dziurka M., Kowalczyk E., Szarski W., Czyczyło-Mysza I. 2025

Impact of soil drought on yield and leaf sugar content in wheat: Genotypic and phenotypic relationships compared using a doubled haploid population

International Journal of Molecular Sciences, 26: 7833

PDF

Jakovljević D., Warchoł M., Skrzypek E. 2025

Rosmarinic acid as bioactive compound: Molecular and physiological aspects of biosynthesis with future perspectives

Cells, 14: 850

PDF

Dziurka K., Dziurka M., Czyczyło-Mysza I., Laskoś K., Noga-Szyrszeń A., Skrzypek E., Warchoł M., Muszyńska E. 2025

Insight into primary and secondary metabolites of 21-day-old haploid oat embryos

Journal of Plant Growth Regulation, 44: 4396–4407

PDF

Kopeć P., Laskoś K., Płażek A. 2025

Culture of flower buds and ovaries in Miscanthus × giganteus

Agronomy, 15: 962

PDF

Jakovljević D., Warchoł M., Kula-Maximenko M., Skrzypek E. 2025

Toward routine basil (Ocimum basilicum L.) callus culture analysis using non-destructive Raman spectroscopy

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 340: 126326

PDF

Matera A., Warchoł M., Simlat M. 2025

Effect of the Pantoea vagans strain SRS89 on carrot (Daucus carota subsp. sativus L.) seed germination and plant growth under saline conditions

South African Journal of Botany, 180:415-427

PDF

Ptak A., Szewczyk A., Simlat M. , Pawłowska B., Warchoł M. 2024

LED light improves shoot multiplication, steviol glycosides and phenolic compounds biosynthesis in Stevia rebaudiana Bertoni in vitro culture

Scientific Reports, 14: 30860

PDF

Juzoń-Sikora K., Laskoś K., Warchoł M., Czyczyło-Mysza I., Dziurka K., Grzesiak M., Skrzypek E. 2024

Water Relations and Physiological Traits Associated with the Yield Components of Winter Wheat (Triticum aestivum L.)

Agriculture, 14: 1887

PDF

Skrzypek E., Jakovljević D., Warchoł M. 2024

Attempts to regeneration of oat (Avena sativa L.) plants via androgenesis: Influence of pre-treatment and media composition

Acta Societatis Botanicorum Poloniae, 93: 1-13

PDF

Grela M., Czyczyło-Mysza I., Skrzypek E. 2024

Crucial factors influencing the efficiency of androgenesis in oat (Avena sativa L.) through anther and microspore cultures

Agronomy, 14: 2394

PDF

Konieczna W., TurkanS., Warchoł M., Skrzypek E., Dąbrowska G.B., Mierek-Adamska A. 2024

The contribution of Trichoderma viride and metallothioneins in enhancing the seed quality of Avena sativa L. in Cd-contaminated soil

Foods, 13: 2469

PDF

Laskoś K., Czyczyło-Mysza I., Waligórski P., Dziurka K., Skrzypek E., Warchoł M., Juzoń-Sikora K., Janowiak F., Dziurka ., Grzesiak M., Grzesiak S., Quarrie S., Marcińska I. 2024

Characterising biological and physiological drought signals in diverse parents of a wheat mapping population

International Journal of Molecular Sciences, 25: 6573

PDF

Warchoł M., Juzoń-Sikora K., Rančić D., Pećinar I., Warzecha T., Idziak-Helmcke D., Laskoś K., Czyczyło-Mysza I., Dziurka K., Skrzypek E. 2024

Comparative characteristics of oat doubled haploids and oat × maize addition lines: Anatomical features of the leaves, chlorophyll a fluorescence and yield parameters

PLoS ONE, 19: e0298072

PDF

Jakovljević D., Kruszka D., Waligórski P., Warchoł M., Skrzypek E. 2024

Untargeted metabolomic in basil cell cultures – a case study of Ocimum basilicum L. var. minimum Alef.

Physiologia Plantarum, 176: e14203

PDF

See all

Projects

Modeling the impact of climate change-induced multifactorial stress on winter barley deacclimation, leading to the identification of key regulatory genes

2024/55/D/NZ1/02024, 2025 - 2028

Identyfikacja czynników warunkujących indukcję embriogenezy mikrospor u pszenicy zwyczajnej (Triticum aestivum L.)

MRiRW nr 4, 2021 - 2026

Określenie fizjologicznych i biochemicznych wskaźników tolerancji pszenicy ozimej (Triticum aestivum L.) na stres suszy i wysokiej temperatury

MRiRW nr 3, 2021 - 2026

The role of oilseed press cake extracts in regulating photosynthesis, water management, and the antioxidant system in winter wheat and winter rye under soil drought conditions

2025/09/X/NZ1/00988, 2025 - 2026

Oxidative stress and its role in the regulation of embryogenesis in isolated microspore cultures of triticale (x Triticale Wittm.) and barley (Hordeum vulgare L.)

2015/18/M/NZ3/00348, 2016 - 2021

Wykonanie prac badawczo-rozwojowych związanych z opracowaniem nowych zestawów dietetycznych bazujących na niezbędnych, nienasyconych kwasach tłuszczowych oraz dodatków, bazujących na kwasach tłuszczowych z grupy NNKT, do wybranych produktów spożywczych celem polepszenia ich wartości odżywczej i sensorycznej

POIR.02.03.02-12-0039/19-00, 2020 - 2020

Protocol for effective doubled haploids production by androgenesis for polish breeding materials of wheat (Triticum aestivum L.)

Tango2/340285/NCBR/2017, 2017 - 2020

Is 4-chloro-indolyl-3-acetic acid the death hormone in generative oat organs?

2017/1/x/NZ9/01309 MINIATURA, 2017 - 2018

Obtaining of oat doubled haploid lines by wide crossing method, and the identification of partial hybrids

PBS3/B8/17/2015, 2015 - 2018

Identification of factors determining the efficiency of doubled haploids production in rye (Secale cereale L.) through androgenesis and distant crosses

MRiRW, nr 84, 2015 - 2018

Identification of factors which determine drought and freezing tolerance in barley (Hordeum vulgare L.)

MRiRW, nr 26, 2014 - 2018

Rozwój izolowanego bielma: problemy morfogenezy i gromadzenia skrobi

UMO-2012/07/B/NZ9/01325, 2013 - 2017

Development of an efficient method of oat doubled haploid production

HOR hn-801-1/13, 2013 - 2013

Development of an efficient method of oat doubled haploid production

HOR hn-801-4/12, 2012 - 2012

Poliamines and phenolics activity in resistance reaction for soil drought of legume plants cultivated for proteins

621/N-COST/09/2010/0, 2010 - 2012

Signalling transduction in resistance to snow mould Microdochium nivale induced by cold

595/N-COST/2009/0605, 2010 - 2012

Optymalization methods for obtaining of doubled haploid oat Avena sativa L.

4521/B/P01/2010/38, 2010 - 2012

Physico-chemical indicators of interactions of polyamines with other antioxidants in conditions of drought and salinity stress

556/N-COST/2009/0, 2009 - 2012

The search for physiological indicators of increased tolerance of plants pea and yellow lupine to drought

HOR hn-801-2/11, 2011 - 2011

Genetic and physiological markers of drought tolerance in spring wheat

192/N-COST/2008/0, 2008 - 2011

See all