Department of Biotechnology

Leader Prof. dr hab. inż. Ilona Czyczyło-Mysza

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Scientific staff

SCIENTIFIC INTRESTS:

Androgenesis and wide crossing of cereals
Endogenous phytohormones regulating the conversion of haploid oat embryos into plants
Physiological and cytogenetic characterization of oat x maize hybrids
Phenotyping and identification of QTLs involved in physiological-biochemical processes, yield and tolerance to abiotic and biotic stresses in crop plants
Genetic, biochemical and physiological determinants of rye response to drought stress with reference to the influence of epicuticular wax structure
Optimization of regeneration methods for chosen endangered species and crop plants, with particular emphasis on legumes and cereals

SCIENTIFIC METHODOLOGY:

In vitro culture techniques: callus induction and regeneration, cell suspension cultures, protoplasts isolation, somatic and gametic embryogenesis, production of cereal doubled haploids
Histological and cytological analyses
Analysis of plant tissues ploidy by flow cytometry (MACS Quant, Miltenyi Biotec)
Hydroponic cultures of chosen crop plants
Plant phenotyping and QTL analysis
Spectrophotometic/chromatographic analysis of phenolics, polyamines, carbohydrates, proteins, plant pigments, antioxidative enzymes activities
Measurement of chlorophyll fluorescence (Handy PEA, Hansatech)
Measurement of chlorophyll content (SPAD, Konica Minolta)
Determination of fatty acids and wax fraction components by gas chromatography

ACHIEVEMENTS:

Obtaining the polish patent no 169824 entitled: Method for culture of fungi containing chitin
Showing that winter wheat callus is not capable of the vernalisation process, however it transfers the ability of generative development into regenerated plants
Detection that the repression of genes responsible for the state of generative induction of winter wheat plants occurs in the course of the process of gamete formation during meiosis
Detection of aromatic cytokinin from BAP group in winter wheat, and correlation of their endogenous content in immature inflorescences and embryos with their ability of generative development
Enriching the map of wheat with 1445 DArT markers, which allowed to dense the map based on SSR, RFLP and AFLP markers for 472 unique loci. Identification of gene candidates for mapped quantitative trait loci (QTLs) of yield components, chlorophyll fluorescence kinetics parameters and photosynthetic pigments
Implementation of oat doubled haploid lines obtained using the wide crossing method in breeding programs
Identification and characterisation of oat x maize hybrids
Poor conversion of oat haploid embryos into plants was due to their immaturity, their irregular morphology and anatomy as well as low content of endogenous auxins suggest disturbances in auxin signaling
Rye's response to drought depends not only on the amount and structure of wax, but also on the interactions between the two, the genetic background of plants, and environmental conditions

AWARDS:

2014 - Distinction of the Minister of Agriculture and Rural Development for outstanding national achievement relevant to the implementation of progress in agricultural practice and realization, implementation and dissemination of the results of scientific work entitled: "Getting the doubled haploid lines of oat (Avena sativa L.) by wide crossing method" awarded to the team of authors: IPP PAS: dr hab. inż. Edyta Skrzypek, prof. dr hab. Izabela Marcińska, dr hab. inż. Ilona Czyczyło-Mysza, dr inż. Kinga Dziurka, dr Agata Nowakowska oraz PB Strzelce Sp. z o.o., Gr. IHAR: dr inż. Zygmunt Nita, inż. Krystyna Werwińska
2021 - Distinction of the Minister of Agriculture and Rural Development granted for outstanding national achievement in the implementation of progress in agriculture and agricultural markets entitled: "Development of a dietary products sets and food products with high pro-health properties" awarded to the IPP PAS team: dr hab. inż. I. Czyczyło-Mysza (team leader), prof. dr hab. inż. E. Pisulewska, prof. dr hab. inż. F. Janowiak, dr hab. P. Waligórski, dr hab. inż. A. Janeczko, mgr inż. K. Laskoś, dr inż. Sz. Polaszczyk, mgr I. Sadura
2022 - Third Degree Young Researcher Award for mgr inż. K. Laskoś awarded by the Polish Botanical Society for the achievement: Laskoś K., CzyczyłoMysza I.M., Dziurka M., Noga A., Góralska M., Bartyzel J., Myśków B. (2021). Correlation between leaf epicuticular wax composition and structure, physiobiochemical traits and drought resistance in glaucous and non-glaucous near-isogenic lines of rye. Plant J, 108: 93-119

Publications

Grela M., Czyczyło-Mysza I., Skrzypek E. 2024

Crucial factors influencing the efficiency of androgenesis in oat (Avena sativa L.) through anther and microspore cultures

Agronomy, 14: 2394

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Konieczna W., TurkanS., Warchoł M., Skrzypek E., Dąbrowska G.B., Mierek-Adamska A. 2024

The contribution of Trichoderma viride and metallothioneins in enhancing the seed quality of Avena sativa L. in Cd-contaminated soil

Foods, 13: 2469

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Laskoś K., Czyczyło-Mysza I., Waligórski P., Dziurka K., Skrzypek E., Warchoł M., Juzoń-Sikora K., Janowiak F., Dziurka ., Grzesiak M., Grzesiak S., Quarrie S., Marcińska I. 2024

Characterising biological and physiological drought signals in diverse parents of a wheat mapping population

International Journal of Molecular Sciences, 25: 6573

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Warchoł M., Juzoń-Sikora K., Rančić D., Pećinar I., Warzecha T., Idziak-Helmcke D., Laskoś K., Czyczyło-Mysza I., Dziurka K., Skrzypek E. 2024

Comparative characteristics of oat doubled haploids and oat × maize addition lines: Anatomical features of the leaves, chlorophyll a fluorescence and yield parameters

PLoS ONE, 19: e0298072

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Jakovljević D., Kruszka D., Waligórski P., Warchoł M., Skrzypek E. 2024

Untargeted metabolomic in basil cell cultures – a case study of Ocimum basilicum L. var. minimum Alef.

Physiologia Plantarum, 176: e14203

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Turkan S., Kulasek M., Zienkiewicz A., Mierek-Adamska A., Skrzypek E., Warchoł M., Szydłowska-Czerniak A. , Bartoli J., Field B., Dąbrowska G.B. 2024

Guanosine tetraphosphate (ppGpp) is a new player in Brassica napus L. seed development

Food Chemistry, 436: 137648

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Warchoł M., Skrzypek E., Juzoń-Sikora K., Jakovljević D. 2023

Oat (Avena sativa L.) In vitro cultures: prospects and challenges for breeding

Agronomy, 13: 2604

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Ptak A., Szewczyk A., Simlat M., Błażejczak A., Warchoł M. 2023

Meta-Topolin-induced mass shoot multiplication and biosynthesis of valuable secondary metabolites in Stevia rebaudiana Bertoni bioreactor culture

Scientific Reports, 13: 15520

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Warzecha T., Bocianowski J., Warchoł M., Bathelt R., Sutkowska A., Skrzypek E. 2023

Effect of soil drought stress on selected biochemical parameters and yield of oat × maize addition (OMA) lines

International Journal of Molecular Sciences, 24: 13905

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Jakovljević D., Skrzypek E., Stanković M., Warchoł M. 2023

Phytochemical diversity and biological activity of basil (Ocimum L.) secondary metabolites produced in vitro.

In: Kumar, N., S. Singh, R. (eds) Biosynthesis of Bioactive Compounds in Medicinal and Aromatic Plants. Food Bioactive Ingredients. Springer, Cham

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Proietti S., Falconieri G. S., Bertini L., Pascale A., Bizzarri E., Morales-Sanfrutos J., Sabidó E., Ruocco M., Monti M. M., Russo A., Dziurka K., Ceci M., Loreto F., Caruso C. 2023

Beauveria bassiana rewires molecular mechanisms related to growth and defense in tomato

Journal of Experimental Botany, 74: 4225–4243

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Śmigała-Lasota M., Dziurka K., Dąbrowska A., Winiarczyk K. 2023

Development of the male and female gametophyte, fertilization, and assessment of germination and regulation of dormancy in Iris aphylla L. seeds

Acta Scientiarum Polonorum Hortorum Cultus, 22: 29–40

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Laskoś K., Czyczyło-Mysza I. 2023

Wosk epikutykularny liści jako cecha warunkująca odporność na suszę u zbóż

Postępy Biochemii, 69: 104-112

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Hura T., Hura K., Dziurka K., Ostrowska A., Urban K. 2023

Cell dehydration of intergeneric hybrid induces subgenome-related specific responses

Physiologia Plantarum, 175: e13855

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Grzesiak M., Maksymowicz A., Hura K., Dziurka K., Ostrowska A., Grzesiak S. 2023

Separate or combined effects of soil compaction and/or drought on gas exchange, chlorophyll fluorescence and physiological traits of maize (Zea mays L.) hybrids

Journal of Agronomy and Crop Science, 209: 689-704

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Muszyńska E., Dziurka K., Labudda M. 2023

What makes the life of stressed plants a little easier? Defense mechanisms against adverse conditions

Plants, 12: 1040

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Konieczna W., Warchoł M., Mierek-Adamska A., Skrzypek E., Waligórski P., Piernik A., Dąbrowska G.B. 2023

Changes in physio-biochemical parameters and expression of metallothioneins in Avena sativa L. in response to drought

Scientific Reports, 13: 2486

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Konieczna W., Mierek-Adamska A., Warchoł M., Skrzypek E., Dąbrowska G.B. 2023

The involvement of metallothioneins and stress markers in response to osmotic stress in Avena sativa L.

Journal of Agronomy and Crop Science, 209: 371-389

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Morańska E., Simlat M., Warchoł M., Skrzypek E., Waligórski P., Laurain-Mattar D., Spina R., Ptak A. 2023

Phenolic acids and Amaryllidaceae alkaloids profiles in Leucojum aestivum L. in vitro plants grown under different light conditions

Molecules, 28: 1525

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Warzecha T., Bathelt R., Skrzypek E., Warchoł M., Bocianowski J., Sutkowska A. 2023

Studies of oat-maize hybrids tolerance to soil drought stress

Agriculture, 13: 243

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Projects

Określenie fizjologicznych i biochemicznych wskaźników tolerancji pszenicy ozimej (Triticum aestivum L.) na stres suszy i wysokiej temperatury

MRiRW nr 3, 2021 - 2026

Identyfikacja czynników warunkujących indukcję embriogenezy mikrospor u pszenicy zwyczajnej (Triticum aestivum L.)

MRiRW nr 4, 2021 - 2026

Oxidative stress and its role in the regulation of embryogenesis in isolated microspore cultures of triticale (x Triticale Wittm.) and barley (Hordeum vulgare L.)

2015/18/M/NZ3/00348, 2016 - 2021

Wykonanie prac badawczo-rozwojowych związanych z opracowaniem nowych zestawów dietetycznych bazujących na niezbędnych, nienasyconych kwasach tłuszczowych oraz dodatków, bazujących na kwasach tłuszczowych z grupy NNKT, do wybranych produktów spożywczych celem polepszenia ich wartości odżywczej i sensorycznej

POIR.02.03.02-12-0039/19-00, 2020 - 2020

Protocol for effective doubled haploids production by androgenesis for polish breeding materials of wheat (Triticum aestivum L.)

Tango2/340285/NCBR/2017, 2017 - 2020

Is 4-chloro-indolyl-3-acetic acid the death hormone in generative oat organs?

2017/1/x/NZ9/01309 MINIATURA, 2017 - 2018

Obtaining of oat doubled haploid lines by wide crossing method, and the identification of partial hybrids

PBS3/B8/17/2015, 2015 - 2018

Identification of factors determining the efficiency of doubled haploids production in rye (Secale cereale L.) through androgenesis and distant crosses

MRiRW, nr 84, 2015 - 2018

Identification of factors which determine drought and freezing tolerance in barley (Hordeum vulgare L.)

MRiRW, nr 26, 2014 - 2018

Rozwój izolowanego bielma: problemy morfogenezy i gromadzenia skrobi

UMO-2012/07/B/NZ9/01325, 2013 - 2017

Development of an efficient method of oat doubled haploid production

HOR hn-801-1/13, 2013 - 2013

Physico-chemical indicators of interactions of polyamines with other antioxidants in conditions of drought and salinity stress

556/N-COST/2009/0, 2009 - 2012

Poliamines and phenolics activity in resistance reaction for soil drought of legume plants cultivated for proteins

621/N-COST/09/2010/0, 2010 - 2012

Optymalization methods for obtaining of doubled haploid oat Avena sativa L.

4521/B/P01/2010/38, 2010 - 2012

Development of an efficient method of oat doubled haploid production

HOR hn-801-4/12, 2012 - 2012

Signalling transduction in resistance to snow mould Microdochium nivale induced by cold

595/N-COST/2009/0605, 2010 - 2012

Genetic and physiological markers of drought tolerance in spring wheat

192/N-COST/2008/0, 2008 - 2011

The search for physiological indicators of increased tolerance of plants pea and yellow lupine to drought

HOR hn-801-2/11, 2011 - 2011

Development of an efficient method of oat doubled haploid production

HOR hn-801-3/11, 2011 - 2011

Improvement of grain dietary value and drought resistance of oat plants by obtaining doubled haploid lines

N R12 002904, 2008 - 2011

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